Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

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5.3K Views

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11:24 min

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May 14th, 2019

DOI :

10.3791/59578-v

May 14th, 2019

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Siming Liu¹^,², Mikako Harata¹^,², Joseph A. Promes¹^,², Anthony J. Burand²^,³, James A. Ankrum²^,³, Yumi Imai¹^,²

¹Department of Internal Medicine, Carver College of Medicine, University of Iowa, ²Fraternal Order of Eagles Diabetes Research Center, University of Iowa, ³Roy J. Carver Department of Biomedical Engineering, University of Iowa

Podsumowanie

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Lentiviral Gene Silencing

Gene Expression Modulation

Human Pseudoislet

SHR8 Transduction

Islet Function Preservation

Low Attachment Plates

Cell Culture Protocol

Proteolytic Enzyme

Collagenolytic Enzyme

Centrifugation

PBS Wash

Fetal Bovine Serum

Cell Dissociation Technique

Islet Equivalent Concentration

Rozdziały w tym wideo

0:04

Title

0:42

Overnight Culture of Human Islets for Recovery After Shipment

1:32

Preparation of Single Cell Suspension from Human Islets

4:03

Pseudoislet Formation and Transduction by Lentivirus

8:44

Results: Morphological and Functional Studies Using Gene Modified Human Pseudoislets

9:53

Conclusion

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