Overnight Culture of Human Islets for Recovery After Shipment
1:32
Preparation of Single Cell Suspension from Human Islets
4:03
Pseudoislet Formation and Transduction by Lentivirus
8:44
Results: Morphological and Functional Studies Using Gene Modified Human Pseudoislets
9:53
Conclusion
Transcription
It's difficult to modulate the gene expression in human islets, also greater decline of the functioning human islets pose major challenges after modulation of gene expression. By combining the lentivirus mediated SHR8 transduction and the formatio
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A protocol to create gene modified human pseudoislets from dispersed human islet cells that are transduced by lentivirus carrying short hairpin RNA (shRNA) is presented. This protocol utilizes readily available enzyme and culture vessels, can be performed easily, and produces genetically modified human pseudoislets suitable for functional and morphological studies.