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Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates

5.4K Views

11:24 min

May 14th, 2019

DOI :

10.3791/59578-v

May 14th, 2019


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Lentiviral Gene Silencing

Chapters in this video

0:04

Title

0:42

Overnight Culture of Human Islets for Recovery After Shipment

1:32

Preparation of Single Cell Suspension from Human Islets

4:03

Pseudoislet Formation and Transduction by Lentivirus

8:44

Results: Morphological and Functional Studies Using Gene Modified Human Pseudoislets

9:53

Conclusion

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