Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

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11.7K Views

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09:23 min

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June 30th, 2019

DOI :

10.3791/59703-v

June 30th, 2019

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Masanori Asai*¹, Yanwen Li*¹, Jasmeet Singh Khara¹^,², Camilla A. Gladstone¹, Brian D. Robertson³, Paul R. Langford*¹, Sandra M. Newton*¹

¹Section of Pediatric Infectious Diseases and Allergy, Department of Medicine, Imperial College London, ²Department of Pharmacy, National University of Singapore, ³MRC Centre for Molecular Bacteriology and Infection, Department of Medicine, Imperial College London

Podsumowanie

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Galleria Mellonella

Infection Model

Mycobacterium Tuberculosis

Tuberculosis Research

Antimycobacterial Drug Efficacy

Innate Immunity

Host pathogen Interaction

Mammalian Models

BCG Lux Culture

Bioluminescence Measurement

Middlebrook 7H9 Broth

Colony forming Units

PBS T Washing

Bacterial Pathogens

Rozdziały w tym wideo

0:04

Title

0:59

Preparation of M. bovis BCG lux for Infection

3:09

Infecting G. mellonella with BCG lux

5:23

Measuring the In Vivo Burden of BCG lux in G. mellonella

7:10

Results: BCG lux Dose Dependent Virulence

8:31

Conclusion

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