Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis
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10:35 min
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January 30th, 2020
DOI :
January 30th, 2020
•Rozdziały w tym wideo
0:04
Introduction
0:27
Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest
2:20
Transformation of C. trachomatis with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange
5:54
Clonal Isolation of Green-only C. trachomatis Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution
7:38
Transformation of C. trachomatis FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette
8:42
Results: FLAEM Enables Markerless Gene Deletion in C. trachomatis and alleviates Cassette-induced Polar Effects
9:47
Conclusion
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