Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest
2:20
Transformation of C. trachomatis with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange
5:54
Clonal Isolation of Green-only C. trachomatis Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution
7:38
Transformation of C. trachomatis FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette
8:42
Results: FLAEM Enables Markerless Gene Deletion in C. trachomatis and alleviates Cassette-induced Polar Effects
9:47
Conclusion
필기록
Floxed cassette allelic exchange mutagenesis is an important method because gene deletion is a valuable tool that can be utilized to understand the function of gene products. The main advantage of this technique is that it allows targeted gene del
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Described here is a method for targeted, markerless gene deletion in Chlamydia trachomatis using floxed cassette allelic exchange mutagenesis, FLAEM.