A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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2.6K Views
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08:53 min
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September 15th, 2021
DOI :
September 15th, 2021
•Rozdziały w tym wideo
0:04
Introduction
0:31
Cotransfection of HeLa Cells with the Reporter and U1 Small Nuclear RNA (snRNA) Plasmids
2:00
32P-Labeling of Oligonucleotides
2:52
Analysis of the Spliced Reporter Transcripts by Primer Extension
3:53
Analysis of the Spliced Reporter Transcripts by Fluorescent RT-PCR
5:08
Analysis of Variant U1 snRNA Expression by RT-qPCR
5:38
Setup and Running of Urea-PAGE Gels
6:27
Results: Reporter Based Splicing Efficiency of U1 snRNA Constructs
8:09
Conclusion
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