Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives

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3.9K Views

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06:09 min

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November 8th, 2021

DOI :

10.3791/63116-v

November 8th, 2021

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Kristina Xiao Liang*¹^,², Anbin Chen*¹^,²^,³^,⁴, Cecilie Katrin Kristiansen¹^,², Laurence A. Bindoff¹^,²

¹Department of Clinical Medicine (K1), University of Bergen, ²Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, ³Department of Neurosurgery, Qilu Hospital and Institute of Brain and Brain-Inspired Science, Cheeloo College of Medicine, Shandong University, ⁴Shandong Key Laboratory of Brain Function Remodeling

Podsumowanie

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Flow Cytometry

Mitochondrial Parameters

Human Induced Pluripotent Stem Cells

Neuro And Glial Derivatives

Staining Solutions

Cell Dissociation Reagent

PBS Wash

Centrifugation

Therapeutic Screening

Neurodegenerative Diseases

Cell Culture Protocol

Paraformaldehyde Fixation

Permeabilization

Blocking Buffer

Rozdziały w tym wideo

0:04

Introduction

0:54

Flow Cytometry Measurement of Mitochondrial Volume, MMP, and Mitochondrial ROS in Live Cells

2:26

Flow Cytometry Measurement of MRC Complex Subunits and TFAM in Fixed Cells

3:49

Flow Cytometry Analysis

4:14

Results: Flow Cytometry Investigation of iPSC-Derived Neural Cells

5:07

Conclusion

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