Autofluorescence Imaging to Evaluate Cellular Metabolism

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4.0K Views

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07:36 min

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November 15th, 2021

DOI :

10.3791/63282-v

November 15th, 2021

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Anna Theodossiou¹, Linghao Hu¹, Nianchao Wang¹, Uyen Nguyen¹, Alex J. Walsh¹

¹Department of Biomedical Engineering, Texas A&M University-College Station

Podsumowanie

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Autofluorescence Imaging

Autofluorescence Microscopy

Live Samples

Multi photon Fluorescence Microscope

Experimental Procedure

Imaging Parameters

Fluorescence Lifetime Imaging FILM

NAPDH

Laser Power Adjustment

Rozdziały w tym wideo

0:04

Introduction

1:04

Multiphoton Fluorescence Lifetime Imaging (FLIM) of Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) and Flavin Adenine Dinucleotide (FAD)

2:13

Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) Imaging

3:35

Flavin Adenine Dinucleotide (FAD) Imaging

4:45

Cyanide Experiment Preparation

5:28

Results: The Fluorescence Imaging of MCF-7 Cells Before and After Cyanide Treatment

7:05

Conclusion

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