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Generating Genetically Modified Plasmodium berghei Sporozoites (Video) | JoVE

DOI :

10.3791/64992-v

May 5th, 2023

May 5th, 2023

787 Views

1Center for Tropical and Emerging Global Diseases, University of Georgia, 2Department of Cellular Biology, University of Georgia

Malaria is transmitted through inoculation of the sporozoite stage of Plasmodium by infected mosquitoes. Transgenic Plasmodium has allowed us to understand the biology of malaria better and has contributed directly to malaria vaccine development efforts. Here, we describe a streamlined methodology to generate transgenic Plasmodium berghei sporozoites.

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