Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

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06:38 min

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December 8th, 2023

DOI :

10.3791/65138-v

December 8th, 2023

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Praapti Jayaswal¹, Mohd Ilyas¹, Kuljit Singh¹^,², Saurabh Kumar¹^,³, Lovely Sisodiya¹, Sapna Jain¹, Rahul Mahlawat¹, Nishant Sharma¹^,⁴, Vishal Gupta¹, Krishnamohan Atmakuri¹

¹Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, ²Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, ³ICAR-Research Complex for Eastern Region, ⁴Public Health Research Institute, Rutgers University

Podsumowanie

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Mycobacterial Extracellular Vesicles

Recombinant EVs

Vaccine Candidates

Mycobacterium Smegmatis

Sautons Media

Centrifugation

Optical Density

Bacterial Culture Incubation

Tween 80

Enrichment Method

Rozdziały w tym wideo

0:00

Introduction

0:42

Growing Mycobacterium smegmatis

2:31

Enrichment of Mycobacterium smegmatis EVs by Density Gradient Centrifugation

5:24

Results: Verifying Enrichment of MsmEVs Containing the EsxA Protein of M. tuberculosis

6:19

Conclusion

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