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JoVE Journal

Immunology and Infection

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Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

19.5K Views

15:01 min

September 24th, 2011

September 24th, 2011

19,487 Views

0:05

Title

1:50

Introduction

2:42

Preparation of Chemoattractant in Agarose Gel

4:30

Preparation of Cremaster Muscle for Intravital Microscopy

10:14

Cell Tracking Using ImageJ

12:18

Analysis of Neutrophil Recruitment Parameters

We describe a protocol of brightfield intravital microscopy for measuring dynamic neutrophil-endothelial cell interactions during neutrophil recruitment in response to the source of a neutrophil chemoattractant in vivo. Neutrophil intraluminal crawling, transendothelial migration and chemotaxis in mouse cremaster muscle tissue are visualized with time-lapsed video photography and tracked with ImageJ.

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