February 21st, 2013
•The mechanical characteristics of endothelial glycocalyx were measured by indentation using micron sized spheres on AFM cantilevers. Endothelial cells were cultured in a custom chamber under physiological flow conditions to induce glycocalyx expression. Data were analyzed using a thin film model to determine the glycocalyx thickness and modulus.
Tags
Vídeos Relacionados
Bacterial Immobilization for Imaging by Atomic Force Microscopy
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
A Novel Method for Localizing Reporter Fluorescent Beads Near the Cell Culture Surface for Traction Force Microscopy
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology
Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification
A Millimeter Scale Flexural Testing System for Measuring the Mechanical Properties of Marine Sponge Spicules
Biaxial Mechanical Characterizations of Atrioventricular Heart Valves
Application of Atomic Force Microscopy to Detect Early Osteoarthritis
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados