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Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

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10:57 min

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February 16th, 2015

February 16th, 2015

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9,634 Views

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Title

1:44

Protein Degradation Mutant Identification Using a Yeast Growth Assay

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Biochemical Confirmation of Protein Degradation Defects

8:29

Results: Ubiquitin Ligase-dependent Degradation of an Unstable Protein Fused to a Metabolic Reporter

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Conclusion

This article describes a yeast growth-based assay for the determination of genetic requirements for protein degradation. It also demonstrates a method for rapid extraction of yeast proteins, suitable for western blotting to biochemically confirm degradation requirements. These techniques can be adapted to monitor degradation of a variety of proteins.

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