April 16th, 2015
•In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.
Tags
Vídeos Relacionados
Neutrophil Extracellular Traps: How to Generate and Visualize Them
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
Immunofluorescence Imaging of Neutrophil Extracellular Traps in Human and Mouse Tissues
Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples
Real-Time High Throughput Technique to Quantify Neutrophil Extracellular Traps Formation in Human Neutrophils
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados