Odor Delivery Cartridge for Type IV Antennal Trichoid Sensillum (AT4) Recording
1:58
Preparing Drosophila for Electrophysiological Recordings while Stimulating AT4
3:46
Taking Electrophysiology Recording of AT4 Sensillum Activity
6:06
Results: Isometric Sensitivity of Or47b Neurons to Palmitoleic Acid
7:06
Conclusion
TRANSCRIÇÃO
The overall goal of this technique is to present odorants of low volatility effectively to olfactory receptor neurons and drosophila for electrophysiological recording. Those neurons are housed in the trichoid sensilla and respond to long chain cu
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The overall goal of this protocol is to demonstrate how to present odorants of low volatility for single-sensillum recording from Drosophila olfactory receptor neurons that respond to long-chain cuticular pheromones.