Polyacrylamide Gel Electrophoresis (PAGE) Analysis
6:06
Results: Representative Nascent Protein Expression Analyses
7:31
Conclusion
TRANSCRIÇÃO
This method can help answer key questions in various metabolic disease research fields about molecular alterations of hepatic energy and protein biosynthesis in obesity, non-alcoholic fatty liver, and type two diabetes. The main advantages of this
Sign in or start your free trial to access this content
Here, we present a protocol for the isolation of healthy and functional primary mouse hepatocytes. Instructions for detecting hepatic nascent protein synthesis by non-radioactive labeling substrate were provided to help understand the mechanisms underlying protein synthesis in the context of energy-metabolism homeostasis in the liver.