Preparation of Hep3B Cell Sample for Microscopic Observations
2:59
Observation of Nuclear Translocation of Small-molecule Guests Triggered by UV Light
4:53
Observation of Nuclear Translocation of Small-molecule Guests Triggered by Two-photon NIR Light
5:48
Observation of Nuclear Translocation of Macromolecular Guests Triggered by UV Light
7:04
Cell Viability Assay
8:20
Results: Endosomal Escape and Nuclear Translocation of Guests in Living Cells
9:51
Conclusion
Transcrição
This method can help answer key question in the area of drug delivery, such as how to deliver a drug to a specific cellular-granular. The main advantage of this technique is that nuclear translocation of gas can be induced by light. Demonstrating
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This protocol describes light-triggered nuclear translocation of guests in living cells using caged molecular glue tags. This method is promising for site-selective nuclear-targeting drug delivery.