Results: Representative Serial Block-Face Scanning Electron Microscopy (SBF-SEM) Imaging
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Conclusion
TRANSCRIÇÃO
Serial block-face scanning electron microscopy provides an unprecedented view of three-dimensional tissue ultra structures, and can answer a host of questions previously impossible or exceedingly difficult to pursue. This technique allows the rapi
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This protocol outlines a routine method for using serial block-face scanning electron microscopy (SBF-SEM), a powerful 3D imaging technique. Successful application of SBF-SEM hinges on proper fixation and tissue staining techniques, as well as careful consideration of imaging settings. This protocol contains practical considerations for the entirety of this process.