Results: Analysis of Epithelial Organoid Monolayers
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Conclusion
Transcrição
Organoid-derived monolayers recapitulate patient's intestinal epithelial barrier function and allow to test the effect of therapies aimed at enhancing dysfunction in each patient to identify personalized treatment strategies. Organoid monolayers p
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Here, we describe the preparation of human organoid-derived intestinal epithelial monolayers for studying intestinal barrier function, permeability, and transport. As organoids represent original epithelial tissue response to external stimuli, these models combine the advantages of expandability of cell lines and the relevance and complexity of primary tissue.