April 9th, 2019
•Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.
Vídeos Relacionados
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
Biomimetic Materials to Characterize Bacteria-host Interactions
Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens
Double Labeling Immunofluorescence using Antibodies from the Same Species to Study Host-Pathogen Interactions
Analysis of SAMHD1 Restriction by Flow Cytometry in Human Myeloid U937 Cells
An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions
Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
SOBRE A JoVE
Copyright © 2024 MyJoVE Corporation. Todos os direitos reservados