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JoVE Journal

Immunology and Infection

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Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

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11:06 min

April 1st, 2022

April 1st, 2022

3,870 Views

0:04

Introduction

1:07

Protein Density Calibrations on BSLBs

1:47

Titration of His-Tagged Proteins on 12.5 mol% of DGS-(Ni) NTA-Containing BSLBs

4:02

Measurement of T-cell Output Transfer to BSLBs

8:13

Results: FCM for Absolute Protein Quantification on the Cell Surface

10:17

Conclusion

Transcrição

The study of the output of the immune synapse has been restricted mostly to microscopic methods. BSLBs have expanded our repertoire of tools that can be used to study the output of T cell immune synapses, including flow cytometry, microscopy, prot

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Here we present the protocol for the stepwise reconstitution of synthetic antigen-presenting cells using Bead-Supported Lipid Bilayers and their use to interrogate the synaptic output from activated T cells.

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