Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

4.0K Views

11:06 min

April 1st, 2022

DOI :

10.3791/63130-v

April 1st, 2022


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Bead supported Lipid Bilayers

Chapters in this video

0:04

Introduction

1:07

Protein Density Calibrations on BSLBs

1:47

Titration of His-Tagged Proteins on 12.5 mol% of DGS-(Ni) NTA-Containing BSLBs

4:02

Measurement of T-cell Output Transfer to BSLBs

8:13

Results: FCM for Absolute Protein Quantification on the Cell Surface

10:17

Conclusion

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