Seeding Motoneuron Aggregates in Bioreactor (Day 14)
5:31
Simultaneous Optical Stimulation and Video Recording of Neuromuscular Junction (NMJ) Function (Day 24+)
7:36
Batch Processing and Analysis (Day 24+)
8:34
Results: Stimulation, Recording, and Analysis of NMJ Function
10:31
Conclusion
Transcrição
This protocol describes how to engineer functional 3D in vitro human neuromuscular junctions that can be used to investigate neuromuscular pathologies and test potential therapeutics. This technique uses simultaneous optogenetic stimulation and vi
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We describe a reproducible, automated, and unbiased imaging system for characterizing neuromuscular junction function using human engineered skeletal muscle tissue and optogenetic motoneurons. This system allows for the functional quantification of neuromuscular connectivity over time and detects diminished neuromuscular function caused by neurotoxins and myasthenia gravis patient serum.