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Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction

2.3K Views

11:07 min

April 14th, 2022

DOI :

10.3791/63759-v

April 14th, 2022


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Optogenetic Model

Chapters in this video

0:04

Introduction

0:48

Fabrication of Bioreactors

2:31

Skeletal Muscle Tissue Seeding (Day 3)

3:22

Motoneuron Differentiation (Days 0 - 14)

4:01

Seeding Motoneuron Aggregates in Bioreactor (Day 14)

5:31

Simultaneous Optical Stimulation and Video Recording of Neuromuscular Junction (NMJ) Function (Day 24+)

7:36

Batch Processing and Analysis (Day 24+)

8:34

Results: Stimulation, Recording, and Analysis of NMJ Function

10:31

Conclusion

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