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Isolation of Conditionally Immortalized Mouse Glomerular Endothelial Cells with Fluorescent Mitochondria

1.9K Views

11:49 min

September 13th, 2022

September 13th, 2022

1,938 Views

0:04

Introduction

0:53

Isolation of Mouse Kidney Glomerular Cells

6:08

Isolating the Glomerular Endothelial Cells with CD31‐Coated Beads

9:04

Results: Isolation and Characterization of MitoDendra2‐GECs

11:04

Conclusion

Transcrição

Immortalized glomerular endothelial cells with stable fluorescent mitochondria are a great tool to examine the effect of different stimuli on mitochondrial structure in vitro. The described method in the current article yields a high number of glo

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The article describes the method for isolating conditionally immortalized glomerular endothelial cells from the kidneys of transgenic mice expressing the thermolabile simian virus 40 and photo-activatable mitochondria, PhAMexcised. We describe the procedure for glomeruli isolation from whole kidneys using beads, digestion steps, seeding, and culturing of GECs-CD31 positive.

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