Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

DOI :

10.3791/64949-v

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February 10th, 2023

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777 Views

Huizheng Ge^1*, Linlin Zhang^2,3*, Mengjia Liu^2,3*, Xi Yang¹, Xu Wu¹, Ran Wang^2,3, Zhengde Xie^2,3

¹Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd, ²Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, ³Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences

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In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers.

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