Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

900 Views

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06:22 min

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February 10th, 2023

DOI :

10.3791/64949-v

February 10th, 2023

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Huizheng Ge*¹, Linlin Zhang*²^,³, Mengjia Liu*²^,³, Xi Yang¹, Xu Wu¹, Ran Wang²^,³, Zhengde Xie²^,³

¹Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd, ²Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, ³Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences

Summary

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Standardization

Flow Cytometry

Lymphocytes Detection

Japanese Encephalitis

Laboratory Accreditation

Data Analysis

Performance Check

PMT Voltages

Compensation Controls

Cytometer Configuration

Fluorescence Parameters

Experimental Settings

CST Beads

Chapters in this video

0:05

Introduction

0:57

Create Identical Configuration with the Same Name on Different Cytometers

1:45

Standardizing Experiment Using Cytometer A in the Transferring Lab

3:48

Transferring the Experimental Template to Cytometer B in the Test Method Lab

5:02

Results: A Rapid and Feasible Standardization Method to Transfer Parameters Across Different Flow Cytometers

5:50

Conclusion

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