3D Culture of Organoids from Murine Intestinal Crypts and Single Stem Cell

April 7th 2023



Краткое содержание

We describe a protocol to isolate murine small intestinal crypts and culture intestinal 3D organoids from the crypts. Additionally, we describe a method to generate organoids from a single intestinal stem cell in the absence of a sub-epithelial cellular niche.

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