Results: Organoid Formation from the Dedifferentiating Villi
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Conclusion
Transcript
This method can confirm the organoid-forming capacity of de-differentiating villi epithelium that acquire stem cell markers in vivo. The collection of villi is performed via scraping rather than the EDTA chelation method preventing the complete lo
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The procedure describes isolation of the villi from the mouse intestinal epithelium undergoing dedifferentiation to determine their organoid forming potential.