Sit-to-stand-and-walk from 120% Knee Height: A Novel Approach to Assess Dynamic Postural Control Independent of Lead-limb

Summary

Here, we present a novel protocol to measure positional stability at key events during the sit-to-stand-to-walk using the center-of-pressure to the whole-body-center-of-mass distance. This was derived from the force platform and three-dimensional motion-capture technology. The paradigm is reliable and can be utilized for the assessment of neurologically compromised individuals.

Abstract

Individuals with sensorimotor pathology e.g., stroke have difficulty executing the common task of rising from sitting and initiating gait (sit-to-walk: STW). Thus, in clinical rehabilitation separation of sit-to-stand and gait initiation - termed sit-to-stand-and-walk (STSW) - is usual. However, a standardized STSW protocol with a clearly defined analytical approach suitable for pathological assessment has yet to be defined.

Hence, a goal-orientated protocol is defined that is suitable for healthy and compromised individuals by requiring the rising phase to be initiated from 120% knee height with a wide base of support independent of lead limb. Optical capture of three-dimensional (3D) segmental movement trajectories, and force platforms to yield two-dimensional (2D) center-of-pressure (COP) trajectories permit tracking of the horizontal distance between COP and whole-body-center-of-mass (BCOM), the decrease of which increases positional stability but is proposed to represent poor dynamic postural control.

BCOM-COP distance is expressed with and without normalization to subjects' leg length. Whilst COP-BCOM distances vary through STSW, normalized data at the key movement events of seat-off and initial toe-off (TO1) during steps 1 and 2 have low intra and inter subject variability in 5 repeated trials performed by 10 young healthy individuals. Thus, comparing COP-BCOM distance at key events during performance of an STSW paradigm between patients with upper motor neuron injury, or other compromised patient groups, and normative data in young healthy individuals is a novel methodology for evaluation of dynamic postural stability.

Introduction

Clinical pathologies affecting the sensorimotor systems, for example upper motor neuron (UMN) injury following stroke, lead to functional impairments including weakness, loss of postural stability and spasticity, which can negatively affect locomotion. Recovery can be variable with a significant number of stroke survivors failing to achieve the functional milestones of safe standing or walking^1,2.

The discrete practice of walking and sit-to-stand are common rehabilitative tasks after UMN pathology^3,4, however transitional movements are frequently neglected. Sit-to-walk (STW) is a sequential postural-locomotor task incorporating sit-to-stand (STS), gait initiation (GI), and walking⁵.

Separation of STS and GI, reflective of hesitation during STW has been observed in patients with Parkinson's disease⁶ and chronic stroke⁷, in addition to older unimpaired adults⁸, but not in young healthy individuals⁹. Therefore sit-to-stand-and-walk (STSW) is commonly implemented within the clinical environment and is defined by a pause phase of variable length when standing. However, there are no published protocols to date defining STSW dynamics in a context suitable for patient populations.

Usually in STW studies the initial chair height is 100% of knee height (KH; floor-to-knee distance), foot-width and GI lead-limb are self-selected, arms are constrained across the chest and an ecologically meaningful task context is often absent^5-9. However, patients find rising from 100% KH challenging¹⁰ and frequently adopt a wider foot position compared with healthy individuals¹¹, initiate gait with their affected leg⁷, and use their arms to generate momentum⁷.

To initiate gait, a state change in whole-body movement in a purposeful direction is required¹². This is achieved by uncoupling the whole-body center-of-mass (BCOM: the weighted average of all considered body segments in space¹³) from the center-of-pressure (COP: the position of the resultant ground reaction force (GRF) vector¹⁴). In the anticipatory phase of GI, rapid stereotypical posterior and lateral movement of the COP toward the limb to be swung occurs thereby generating BCOM momentum^12,15. The COP and BCOM are thus separated, with the horizontal distance between them having been proposed as a measure of dynamic postural control¹⁶.

The calculation of COP-BCOM distance requires simultaneous measurement of the COP and BCOM positions. The standard calculation of COP is shown below in equation (1)¹⁷:

(1)

Where M and Force represent moments about the force platform axes and the directional GRF respectively. The subscripts represent axes. The origin is the vertical distance between the contact surface and the origin of the force platform, and is considered to be zero.

The kinematic method of deriving BCOM position involves tracking the displacement of segmental markers. A faithful representation of body-segment motion can be achieved by employing markers clustered on rigid plates placed away from bony landmarks, minimizing soft-tissue-artifact (CAST technique¹⁸). In order to determine BCOM position, individual body segment masses are estimated, based on cadaveric work¹⁹. Three-dimensional (3D) motion system proprietary software uses the coordinate positions of proximal and distal segment locations to: 1) determine segmental lengths, 2) arithmetically estimate segmental masses, and 3) compute segmental COM locations. These models are then able to provide estimates of 3D BCOM position at a given point in time based on the net summation of inter-segmental positions (Figure 1).

Thus, the purpose of this paper is first to present a standardized STSW protocol that is ecologically valid and includes rising from a high seat-height. It has been shown previously that STSW from 120% KH is biomechanically indistinct from 100% KH barring generation of lower BCOM vertical velocities and GRF's during rising²⁰, meaning rising from 120% KH is easier (and safer) for compromised individuals. Second, to derive COP-BCOM horizontal distances to assess dynamic postural control during key milestones and transitions using 3D motion-capture. This approach, which in healthy individuals during STSW is independent of limb-lead²⁰, offers the prospect of functional recovery evaluation. Finally, a preliminary STSW data set representative of young healthy individuals is presented, and intra and inter-subject variability in the group is defined in order to inform comparison with pathological individuals.

Figure 1. 2D BCOM calculation. For simplicity, the example is based on calculating whole-leg COM from a 3-linked mass in 2 dimensions, where coordinates of the respective COM positions (x,y), and segmental masses (m₁, m₂, m₃) are known. Segment masses and location of segmental COM positions, with respect to the laboratory coordinate system (LCS; origin: 0, 0), are estimated by motion analysis system proprietary software using subject body mass and published anthropometric data (see main text). The x and y leg COM position, in this example of the 3-linked mass, is then derived using the formulae shown. Please click here to view a larger version of this figure.

Protocol

The protocol follows the local guidelines for the testing of human participants, defined by London South Bank University research ethics committee approval (UREC1413/2014).

1. Gait Laboratory Preparation

1. Clear the capture volume of unwanted reflective objects that may be misinterpreted as movement markers and eliminate ambient daylight to reduce reflections as appropriate.
2. Turn on motion-capture cameras, proprietary tracking software, force platform amplifiers, and external analogue-to-digital (AD) converter. Allow time for the cameras to initialize.
3. Arrange cameras ensuring that there are at least 2 intersecting axes at the extremes of the capture volume. Ensure individual cameras have optimal exposure and aperture settings by checking individual point-resolution of test markers (e.g., the static calibration frame) within capture volume space (see Reference Appendix A²¹).
4. Mount subject-switch to turn off visual go signal in the midline of the walkway, 6 m in front of the starting position in the direction of travel, on a tripod at subject's navel height. Mount light source (for visual go signal) in the midline of the walkway, 1 m in front of the subject-switch in the direction of travel, on a tripod at subject's canthus height (Figure 2). Arrange the operator light switch in close proximity to the investigator.
5. Arrange force platforms 1 and 2 in parallel for gait-initiation, and force platforms 3 and 4 in a staggered configuration to capture non-dominant lead-limb trials. Then attach force platform covers with removable tape.

Figure 2. Experimental Protocol. This example shows a left-leg lead: Subjects sit on an instrumented stool at 120% knee height (KH) with ankles 10° degrees in dorsiflexion and feet at shoulder width apart orientated forward. On a visual cue, subjects perform 5 trials of STSW leading with their non-dominant limb at self-selected pace terminated by switching off the light. Please click here to view a larger version of this figure.

6. In the proprietary tracking software set capture frequency to 60 Hz and 3D tracking parameters. Specifically, use a prediction error of 20 mm, a maximum residual of 2 mm, minimum trajectory length equivalent to 2 frames, and a maximum frame gap of 10 frames. Go on to identify each of the 8 individual force platform components (z1, z2, z3, z4, x1-2. x3-4, y1-4, y2-3) from each form platform amplifier into the respective analog to digital converter (32 channels in this study).
   1. Ensure all pre-determined calibration settings from each force platform's calibration documentation, scaling factors and analogue channels have been specified (see chapter Project Options; Analogue Boards²¹) and nominate offset to be read during the last 10 frames of capture when unloaded.
7. In the proprietary tracking software, nominate a multiplier to the motion-capture frequency to ensure an adequate analogue sampling frequency. Use a multiplier of 17, yielding an individual force platform sampling frequency of 1,020 Hz.
8. Implement the dynamic wand calibration procedure:
   1. Position the L-shaped reference structure on the floor in the capture volume in preparation for the calibration of 3D space. The long axis of this structure should be pointing in the anterior direction. (see chapter Wand calibration method²¹).
   2. In the Calibration settings page in the Project options dialog, select the calibration 'type' to Wand, with a 750 mm length. Then select coordinate system orientation with positive z-axis pointing upwards and positive y-axis as the long arm (see chapter Calibration²¹). Click OK.
   3. Click the Calibration icon and set the intended length of the calibration capture to 60 sec. Then set a time delay of 5 sec and identify the file directory where the results will be saved. Click OK to commence calibrating.
      Note: The wand procedure uses two calibration objects to calibrate the measurement volume; this is used to maximize the resolution of a large motion capture volume (Figure 3). One is a stationary L-shaped reference structure with four markers attached to it and is used to define the global coordinate system. The other object is a wand, which consists of two markers located a fixed distance from each other. During calibration, the x, y, z orientations of these are tracked with respect to the x, y, z positions of the four static markers on the reference structure; in turn permitting the proprietary software to triangulate, predict and reconstruct the trajectories of the moving markers in 3D space. At the end of this process, each camera will return a residual error of its accuracy.
   4. Move the calibration wand within the calibration volume by rotating and translating the wand around the intended capture volume for the specified 60 sec (see chapter Wand Calibration Method²¹).
   5. Check the Calibration results, accept calibration with individual camera residual errors of < 1.5 mm, click OK.
      Note: If you have force plates there will be a warning reminding you of measuring the force plate position again (since it has most probably changed with the new calibration).

Figure 3. L-Shaped Reference Structure and Wand for Camera Calibration. The L-shaped reference structure remains stationary and has 4 markers attached to it. The wand has two markers attached to it at a fixed distance and is moved, with respect to the reference structure, to create a 3-D calibrated volume of space that is sufficient enough for the intended marker set to pass through. Please click here to view a larger version of this figure.

9. Remove calibration set from capture volume. Locate the force platforms in the calibrated 3D space by placing one 9 mm diameter passive retro-reflective marker in each of the 4 corners of each platform (attention to placement is essential; see chapter Force Plate Location²¹). Obtain a 5 sec recording and proceed to identify each marker and each platform's reference system (PRS) within the 3D space as per proprietary software suggestions.
10. Undertake a dynamic capture using the aforementioned sampling and 3D tracking parameters (1.6) to confirm and sense-check subsequent force magnitudes and directions.
    1. Set up dynamic capture for 15 sec with a 5 sec delay. Once the click to commence capture is initiated, the operator has time to sit on the stool, pause, stand, pause and walk forwards making contact with the force platforms (at this point, there is no need for the operator to have retro-reflective markers attached in situ).
    2. Once the capture has finished, check the direction and magnitudes of ground reaction vectors to ensure configurations of force-platforms are correct. Expect upward and posterior to direction of travel vectors at foot contact, and a maximum vertical force of approximately 1 to 1.5 times body weight.
11. Place height adjustable stool in the midline of the capture volume between force platforms 1 and 2 (Figure 2), then connect a 300 mm diameter pressure seat-mat to the external AD converter.
12. Prepare all passive retro-reflective anatomical markers for fixation by pre attaching individually to one side of double adhesive tape, approximately 15 mm in length (at least 60 cm of double adhesive tape in total per subject) and arrange in an appropriate location ready for application to subject. Include tracking marker clusters and self-securing bandage ready for timely subject application.
    Note: Tracking markers should comprise a minimum of 3 retro-reflective markers arranged in a non-co-linear arrangement, and are placed on body segments (some anatomical markers positioned at estimated joint centers can be used as tracking markers e.g., 1^st and 5^th metatarsals).

2. Subject Preparation

1. Obtain written informed consent from subject who fulfills inclusion/exclusion criteria.
2. Ask subject to change into suitable clothing (cycling shorts, close fitting t-shirt and sports bra as appropriate).
3. Establish dominant lower limb using the kicking-a-ball test²² if the subject is able to safely do so.
4. Measure subject standing height (m) and mass (kg); convert mass to weight (N).
5. With subject standing, measure subject bi-acromial distance (m) using measuring calipers. Lock caliper position to use distance for feet positioning (see 4.5 below).
6. Measure vertical floor-to-knee distance (m) on the dominant limb (in standing); multiply distance by 1.2 to calculate 120% KH distance (m). Adjust stool height to 120% KH. Table 1 summarizes 10 healthy subject characteristics including knee height data.

Table 1: Subject Characteristics. Individual data and mean (±1 SD) across 10 subjects are shown.

7. Prepare the skin areas for marker placement. Shave unwanted body hair as appropriate and use alcohol wipes to remove excess sweat and/or moisturizer to maximize adherence between markers and the skin.
8. Palpate, identify and apply retro-reflective markers to anatomical landmarks of the lower and upper extremities, trunk, head and pelvic segments using double adhesive tape in accordance with the chosen technical frame of reference²³ (Table 2). Go on to apply segmental tracking markers with self-securing bandage.
   Note: In females, if difficulty arises locating the sternal notch marker - place marker over the center of the sports bra garment.

Table 2: Marker-set placement. Markers (anatomical and tracking) based on a previously reported technical frame of reference²³.

9. Ask subject to walk into the capture volume and adopt the anatomical position. At this point the subject must not move until after static capture has been performed due to the inherent problem of estimating the hip joint center over clothing at this anatomical location.

3. Static Capture

1. Instruct subject to stand stationary in the center of the capture volume, assuming the standard anatomical position, with all anatomical and tracking markers in situ.
   Note: In order to reduce soft tissue artifact a static calibration is undertaken with anatomical and tracking markers in situ. The tracking markers are referenced to the anatomical markers, which negates the limitation of assuming that joint centers do not move under the skin. Tracking markers are left in situ for subsequent dynamic trials. This is termed the calibrated anatomical systems technique (CAST)¹⁸.
   1. In order to undertake a short static capture, use the aforementioned sampling and 3D tracking parameters (1.6) and ensure all markers are accounted for in the capture volume by confirming the total number of markers listed in the Unidentified Trajectories panel in 3D real-time mode. This should correspond with the total number of markers that the chosen technical frame of reference requires. Click the record icon to complete a 5 sec capture. Repeat procedure where necessary if markers are missing.
      Note: See section 6 below for the processing of static capture data.
2. Use the position data from the hip-joint-center landmark on the subject dominant side to determine leg-length (distance from hip-joint-center (see 7.1 and Table 3b below) to floor) for distance normalization (see 7.11 below).

4. Familiarization

1. Remove all anatomical-only markers.
2. Instruct subject to sit on the stool with feet on individual force platforms 1 & 2.
3. Instruct subject to stand and then walk forward with the defined leading leg. Adjust the anteroposterior position of the stool until the subject consistently makes central contact with force platforms 3 and 4 during the first 2 steps of gait. Allow repeated practice trials until the subject is comfortable.
4. Mark the front leg position of the stool with tape on the floor surface in order to re-establish stool position.
5. Set up final feet position (Figure 2). Ask subject to sit on the stool with feet on individual force platforms 1 and 2. Adjust shank position on subject's dominant side 10° posterior from vertical using an extendable arm goniometer. Go on to adjust the non-dominant foot equally in line, and then using the locked calipers (see 2.5 above), arrange the inter-foot width to the pre-determined bi-acromial distance accordingly between the lateral foot borders.
6. Adjust the transverse plane orientation of each foot such that each medial foot border is placed in line with the direction of travel.
7. After finally checking alignment, draw around final foot positions using a dry board marker pen onto the removable force platform surface.
8. Use the verbal instruction: "When you see the light come on in front of you, stand up and stop. Mentally count down from 3 to 1, one number at a time. Then, leading with your non-dominant leg, walk at a comfortable pace toward the switch in front of the light and stop. Count mentally from 3 down to 1, one number at a time, and then with your writing hand use the switch to turn off the light".
9. Re-iterate to the subject that they may use their arms naturally, then allow the subject sufficient familiarization to STSW protocol. Familiarization gives the subject as much time as possible to acclimatize to the testing environment ensuring they are able to efficiently accomplish the task without any forced movement that might otherwise impinge on the ecological validity of the experimental paradigm.

5. STSW Dynamic Trials

1. With subject sitting on the stool ready for dynamic trials, first confirm the total number of markers listed in the Unidentified Trajectories panel in 3D real-time mode and that they correspond with the total number of markers that the chosen technical frame of reference requires. Then click the record icon to complete a 15 sec dynamic capture.
2. After 5 sec capture, turn on the operator light-switch and check how the subject responds - that they rise from the stool and pause as instructed, step on to force platforms 3 and 4, and that they stop and turn the light off as instructed within the capture period.
3. Re-set the light switch and check for marker dropouts by accounting for all markers during slow motion playback of trial. Repeat if necessary, otherwise continue to next trial. Go on to capture 5 trials of STSW in each subject.
4. In the event of anatomical markers becoming unattached, re-attach to predetermined skin mark. If tracking markers move, re-attach anatomical markers and repeat static trial - then continue with remaining dynamic trials.

6. Proprietary Tracking Software Post Processing

1. In proprietary tracking software, identify and label all markers from static and dynamic trials (see chapter Manual Identification of Trajectories²¹) and crop unwanted capture by moving the time-slides to the beginning and end of the task. Utilize the "automatic identification of markers", otherwise known as AIM, functionality in the proprietary tracking software to aid labeling (see chapter Generating an AIM Model²¹).
   Note: Labeling of markers is required so that the proprietary and subsequent biomechanics analysis software consistently constructs and calculates the relative trajectory of a rigid body in 3-dimensional space. Use meaningful labeling as shown in Table 2. AIM is subject-specific, but continually updates. With a different subject and in the event of a poor AIM, go on to update AIM by manual labelling. This also applies to the static capture process (see section 3.1.1 above).
2. In the event of marker drop out, that exceeds 10 frames, go on to either locate the missing trajectory in the Unidentified Trajectories panel, or manually gap-fill using the polynomial interpolation function provided by the proprietary software (see chapter Gap Fill Trajectories²¹).
   Note: In some cases marker trajectories are partially absent and gap-filling is a mechanism whereby missing data can be mathematically estimated based on the measured trajectory before and after the missing data.
3. Format and export all static and dynamic trials, in c3d format, for post-processing in biomechanics analysis software.
   Note: Prior to export, exclude all unidentified and empty marker trajectories, specifying de facto labeling, and nominate the last 10 frames for zero force baseline levels for each force plate.

7. Biomechanics Analysis Software Post Processing

1. Build static 13-segment model²³ (feet, shanks, thighs, pelvis, trunk, upper arms, forearms and head (note no hands)).
   Note: The process of model building is fundamental in defining the linked segments based on the static measurement trial and proprietary software instructions were used²⁴. In this protocol the anatomical coordinate systems for each body segment (Table 3a) and joint center locations (Table 3b) are based mainly on Ren et al.²³ with adaptations to avoid functional hip and glenohumeral joint center estimation. Gold standards for all joint center locations remain imaging techniques such as magnetic resonance imaging (MRI), which are unrealistic in most situations. Functional joint center estimations have been utilized; however, there remains the risk that patients with pathology would not be able to move the joint in the requisite planes²⁵. Therefore, for the pelvis regression equations e.g., Davis²⁶ are often used. Here, the CODA pelvis²⁷ was used and is based on work by Bell et al.²⁸, and the glenohumeral joint centers were estimated according to Eames et al.²⁹.

Table 3a: Anatomical Coordinate System for Whole Body Model.

Table 3b: Joint Center Definitions for Whole Body Model.

2. Import the dynamic files and assign the model to each. Confirm accuracy of model building by checking normal visual configuration of segments. In the case of inaccuracy, the operator is advised to go back to the proprietary tracking software files and check sensor image tracking profiles and correct as necessary.
3. Low pass filter kinematic and kinetic data using a 4^th order Butterworth filter with cut-off frequency at 6 Hz and 25 Hz respectively.
4. Average filter light and pressure-mat analogue signals over a 25-frame window.
5. Create force structure for force platforms 1, 2, 3, and 4. Use corner coordinates to create a level-surfaced, rectangular structure encompassing all 4 force platforms (Figure 4).
   Note: A force structure is required³⁰ in order that net COP calculations can be made across the 4 force platforms.
6. Calculate the net COP coordinate signals (x and y) within the laboratory coordinate system (LCS) from the force structure.
   Note: The software performs this by using equations 2a-g below.
   1. Use x and y signals from equations 2f and 2g for net COP position within the LCS.

(2a) Net medio-lateral force 

(2b) Net anterior-posterior force    

(2c) Net vertical force 

(2d) Net platform moment about x-axis 

(2e) Net platform moment about y-axis 

(2f) x-Coordinate of net force application point (COP_x) 

(2g) y-Coordinate of net force application point (COP_y) 
      

Figure 4. Force Structure. Example of a rectangular force structure encompassing 4 force platforms in a right lead-limb orientation. Details of local COP application and dimensions with respect to a laboratory coordinate system (LCS) are shown for force platform 1 as an example. The x, y, z position of the platform reference system (PRS) is offset relative to the LCS where X₁ and Y₁ represent the mediolateral and anteroposterior distances from PRS, respectively. To calculate the individual platform moment about the x-axis, the vertical GRF is multiplied by the sum of the local y COP coordinate and the new PRS-LCS offset y coordinate (Y₁+y₁). The moment about the y-axis coordinate is similarly calculated by multiplying the vertical GRF by the negative sum of the local x COP coordinate and the new PRS-LCS offset x coordinate -(X₁+x₁). The total moment of force about the global force structure is equal to the sum of all of the moments of force, divided by the sum of the individual vertical forces. Net COP X and Y coordinates are thus produced for the force structure within the LCS (equations 2a-g). Please click here to view a larger version of this figure.

7. Using customized pipeline commands, create important movement events within STSW, specifically seat-off, upright, gait initiation onset, first toe-off 1, and 1^st and 2^nd initial contacts (Table 4).

Table 4: Movement Event Definitions.  GI – gait initiation; COP – center-of-pressure; HO1 – first heel-off; TO1 – 1st toe-off, IC1 – 1st initial contact.

8. Using customized pipeline commands calculate the COP-BCOM distance (L) by applying equation 3 at each movement event, where t_i represents a given event.
    
   
   (3)
    
9. Using customized pipeline commands, calculate the maximum COP-BCOM distance (L_max) by applying equation 4 between two events (t_o → t_i).
    
   
   (4)
    
   where: t₀ and t_i represent movement onset and the final time instance of interest respectively, (x_COP(t_i)) is the x coordinate of the COP at time t_i, (x_BCOM(t_i)) is the coordinate of the BCOM at time t_i, and (y_COP(t_i)) and (y_BCOM(t_i)) are the corresponding values for the y coordinates³¹.
10. Extract dependent variables of interest at movement events; COP-BCOM distances at seat-off and first toe-off (TO1) events, and maximum COP-BCOM distances during the 1^st step phase (between TO1 and first initial-contact; IC1) and the 2^nd step phase (between IC1 and IC2) using customized pipeline commands.
11. Normalize intra-subject COP-BCOM distances as a proportion of subject's dominant leg length (see 3.2 above).
12. Export data for statistical analysis using the Copy to Clipboard functionality or by exporting files in other available native formats.

8. Lab-specific Normative Value Calculations

1. Calculate mean (± 1 SD) intra and inter-subject values for both actual COP-BCOM distances and normalized values as proportions of subjects' dominant lower limb length.
2. Calculate coefficients of variation (COV) for mean inter-subject data.
3. Calculate intra-subject variation per event using two-way mixed effects model intra-class correlation coefficients (ICC_3,1), and the measurement error³².

Results

All subjects rose with their feet placed on the twin force platforms, leading with their non-dominant limb as instructed. Normal gait was observed with subjects stepping cleanly onto the other platforms and 3D optical-based motion analysis successfully tracked whole body movement during 5 repeated goal-orientated STSW tasks rising from 120% KH. Simultaneous COP and BCOM mediolateral (ML) and anteroposterior (AP) displacements between seat-off and IC2 (100% STSW cycle) comprising: rise, pa...

Discussion

The sit-to-stand-and-walk (STSW) protocol defined here can be used to test dynamic postural control during complex transitional movement in healthy individuals or patient groups. The protocol includes constraints that are designed to allow subjects with pathology to participate, and the inclusion of switching off the light means it is ecologically valid and goal-orientated. As it has been shown previously that lead-limb and rising from a high (120% KH) seat does not fundamentally affect task dynamics during STSW²⁰

Materials

Name	Company	Catalog Number	Comments
Motion Tracking Cameras	Qualysis  (Qualysis AB Gothenburg, Sweden)	Oqus 300+	n= 8
Qualysis Track Manager (QTM)	Qualysis  (Qualysis AB Gothenburg, Sweden)	QTM 2.9 Build No: 1697	Proprietary tracking software
Force Platform  Amplifier	Kistler Instruments, Hook, UK	5233A	n= 4
Force Platform	Kistler Instruments, Hook, UK	9281E	n= 4
AD Converter	Qualysis  (Qualysis AB Gothenburg, Sweden)	230599
Light-Weight Wooden Walkway Section	Kistler Instruments, Hook, UK	Type 9401B01	n= 2
Light-Weight Wooden Walkway Section	Kistler Instruments, Hook, UK	Type 9401B02	n= 4
4 Point "L-Shaped" Calibration Frame	Qualysis  (Qualysis AB Gothenburg, Sweden)
"T-Shaped" Wand	Qualysis  (Qualysis AB Gothenburg, Sweden)
12 mm Diameter Passive Retro reflective Marker	Qualysis  (Qualysis AB Gothenburg, Sweden)	Cat No: 160181	Flat Base
Double Adhesive Tape	Qualysis  (Qualysis AB Gothenburg, Sweden)	Cat No: 160188	For fixing markers to skin
Height-Adjustable Stool	Ikea, Sweden	Svenerik	Height 43 - 58 cm with ~ 10cm customized height extension option at each leg
Circular (Disc) Pressure Floor Pad	Arun Electronics Ltd, Sussex, UK	PM10	305 mm Diameter, 3 mm thickness, 2 wire
Lower Limb Tracking Marker Clusters	Qualysis  (Qualysis AB Gothenburg, Sweden)	Cat No: 160145	2 Marker clusters, lower body with 8 markers (n= 2)
Upper Limb Tracking Marker Clusters	Qualysis  (Qualysis AB Gothenburg, Sweden)	Cat No: 160146	2 Marker clusters, lower body with 6 markers (n= 2)
Self-Securing Bandage	Fabrifoam, PA, USA		3'' x 5'
Cycling Skull Cap	Dhb	Windslam
Digital Column Scale	Seca	763 Digital Medical Scale w/ Stadiometer
Measuring Caliper	Grip-On	Grip Jumbo Aluminum Caliper - Model no. 59070	24 in. Jaw
Extendable Arm Goniometer	Lafayette Instrument	Model 01135	Gollehon
Light Switch			Custom made
Visual3D Biomechanics Analysis Software	C-Motion Inc., Germantown, MD, USA	Version 4.87