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This manuscript describes a method for continuous video EEG recordings using multiple depth electrodes in neonatal mice undergoing hypoxia-ischemia.
Hypoxia ischemia is the most common cause of neonatal seizures. Animal models are crucial for understanding the mechanisms and physiology underlying neonatal seizures and hypoxia ischemia. This manuscript describes a method for continuous video electroencephalogram (EEG) monitoring in neonatal mice to detect seizures and analyze EEG background during hypoxia ischemia. Use of video and EEG in conjunction allows description of seizure semiology and confirmation of seizures. This method also allows analysis of power spectrograms and EEG background pattern trends over the experimental time period. In this hypoxia ischemia model, the method allows EEG recording prior to injury to obtain a normative baseline and during injury and recovery. Total monitoring time is limited by the inability to separate pups from the mother for longer than four hours. Although, we have used a model of hypoxic-ischemic seizures in this manuscript, this method for neonatal video EEG monitoring could be applied to diverse disease and seizure models in rodents.
Hypoxic ischemic encephalopathy (HIE) is a condition that affects 1.5 in 1000 newborns annually and is the most common cause of neonatal seizures1,2. Infants who survive are at risk for various neurological disabilities such as cerebral palsy, intellectual disability, and epilepsy3,4,5.
Animal models play a critical role in understanding and investigating the pathophysiology of hypoxia ischemia and neonatal seizures6,7. A modified V....
All animal studies were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Virginia.
1. Electrode building/cable building
Seizure semiology
Neonatal hypoxia-ischemia exposure results in both generalized and focal seizures in mice (Figure 1A-C). Video EEG recordings allow electrographic findings to be correlated to behavior on video. These behaviors were scored using a previously published neonatal rodent behavioral seizure score (BSS)16. In addition to BSS, we categorized events based on whether the behavior was focal/unilateral, bila.......
We have presented a model for continuous video-EEG monitoring in neonatal mice during hypoxic-ischemic seizures. Video analysis in conjunction with EEG allows characterization of seizure semiology. Analysis of EEG allows for extraction of power spectrograms and background amplitude analysis.
Correct and careful placement of electrodes is crucial in this protocol, as injury during electrode placement or inaccurate placement can significantly affect results. Assessment of normal baseline EEG act.......
We acknowledge the following funding sources: NIH NINDS – K08NS101122 (JB), R01NS040337 (JK), R01NS044370 (JK), University of Virginia School of Medicine (JB).
....Name | Company | Catalog Number | Comments |
SURGERY | |||
Ball Point Applicator | Metrex Research | 8300-F | i-bond applicator |
Cranioplast (Powder/Resin) | Coltene | H00383 | Perm Reline/Power |
I-Bond | Kulzer GmbH, Germany | ||
LOOK Silk Suture | Surgical Specialities Corporation | SP115 | LOOK SP115 Black Braided Silk Non absorbable surgical suture |
RS-5168 Botvin Forceps | Roboz Surgical Instrument | RS5168 | Forcep for surgery/ligation |
RS-5138 Graefe Forceps | Roboz Surgical Instrument | RS5138 | Forcep for surgery/ligation |
UV light for I-Bond | Blast Lite By First Media | BL778 | UV ligth for I-bond |
Vannas Microdissecting Scissor | Roboz Surgical Instrument | RS5618 | Scissor for ligation |
Vet Bond | 3M Vetbond | 1469SB | Vet Glue |
HYPOXIA | |||
Hypoxidial | Starr Life Science | ||
Oxygen sensor | Medical Products | MiniOxI- oxygen analyzer/sensor for hypoxia rig | |
EEG RECORDING | |||
Female receptacle connector 0.079" | Mill-Max Manufacturing Corp | 832-10-024-10-001000 | Ordered from Digikey |
Grass Amplifier | Natus Neurology Incorporated | Grass Product | |
LabChart Pro | ADI Instruments | Software to run the system | |
Male Socket Connector 0.079" | Mill-Max Manufacturing Corp | 833-43-024-20-001000 | Ordered from Digikey |
Operational Amplifier | Texas Instruments, Dallas, TX, USA | TLC2274CD | TLC2274 Quad Low‐Noise Rail‐to Rail Operational Amplifier |
Operational Amplifier | Texas Instruments, Dallas, TX, USA | TLC2272ACDR | TLC2274 Quad Low‐Noise Rail‐to Rail Operational Amplifier |
Stainless Steel wire | A-M Systems | 791400 | 0.005" Bare/0.008" Coated 100 ft |
Ultra-Flexible Wire | McMaster-Carr | 9564T1 | 36 Gauze wire of various color |
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