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In This Article

  • Summary
  • Abstract
  • Introduction
  • Protocol
  • Results
  • Discussion
  • Disclosures
  • Acknowledgements
  • Materials
  • References
  • Reprints and Permissions

Summary

This study demonstrates the feasibility and safety of developing an autologous pulmonary valve for implantation at the native pulmonary valve position by using a self-expandable Nitinol stent in an adult sheep model. This is a step toward developing transcatheter pulmonary valve replacement for patients with right ventricular outflow tract dysfunction.

Abstract

Transcatheter pulmonary valve replacement has been established as a viable alternative approach for patients suffering from right ventricular outflow tract or bioprosthetic valve dysfunction, with excellent early and late clinical outcomes. However, clinical challenges such as stented heart valve deterioration, coronary occlusion, endocarditis, and other complications must be addressed for lifetime application, particularly in pediatric patients. To facilitate the development of a lifelong solution for patients, transcatheter autologous pulmonary valve replacement was performed in an adult sheep model. The autologous pericardium was harvested from the sheep via left anterolateral minithoracotomy under general anesthesia with ventilation. The pericardium was placed on a 3D shaping heart valve model for non-toxic cross-linking for 2 days and 21 h. Intracardiac echocardiography (ICE) and angiography were performed to assess the position, morphology, function, and dimensions of the native pulmonary valve (NPV). After trimming, the crosslinked pericardium was sewn onto a self-expandable Nitinol stent and crimped into a self-designed delivery system. The autologous pulmonary valve (APV) was implanted at the NPV position via left jugular vein catheterization. ICE and angiography were repeated to evaluate the position, morphology, function, and dimensions of the APV. An APV was successfully implanted in sheep J. In this paper, sheep J was selected to obtain representative results. A 30 mm APV with a Nitinol stent was accurately implanted at the NPV position without any significant hemodynamic change. There was no paravalvular leak, no new pulmonary valve insufficiency, or stented pulmonary valve migration. This study demonstrated the feasibility and safety, in a long-time follow-up, of developing an APV for implantation at the NPV position with a self-expandable Nitinol stent via jugular vein catheterization in an adult sheep model.

Introduction

Bonhoeffer et al.1 marked the beginning of transcatheter pulmonary valve replacement (TPVR) in 2000 as a rapid innovation with significant progress toward minimizing complications and providing an alternative therapeutic approach. Since then, the use of TPVR for treating the right ventricular outflow tract (RVOT) or bioprosthetic valve dysfunction has increased rapidly2,3. To date, the TPVR devices currently available on the market have provided satisfying long-term and short-term results for patients with RVOT dysfunction4,5,6. Furthermore, various types of TPVR valves including decellularized heart valves and stem cell-driven heart valves are being developed and evaluated, and their feasibility has been demonstrated in preclinical large animal models7,8. Aortic valve reconstruction using an autologous pericardium was first reported by Dr. Duran, for which three consecutive bulges of different sizes were used as templates to guide the shaping of the pericardium according to the dimensions of the aortic annulus, with the survival rate of 84.53% at the follow-up of 60 months9. The Ozaki procedure, which is considered a valve repair procedure rather than a valve replacement procedure, involves replacing aortic valve leaflets with the glutaraldehyde-treated autologous pericardium; however, when compared to Dr. Duran's procedure, it improved significantly in measuring the diseased valve with a template to cut fixed pericardium10 and satisfactory results were not only achieved from the adult cases but also pediatric cases11. Currently, only the Ross procedure can provide a living valve substitute for the patient who has a diseased aortic valve with obvious advantages in terms of avoiding long-term anticoagulation, growth potential, and low risk of endocarditis12. But re-interventions may be required for the pulmonary autograft and right ventricle to pulmonary artery conduit after such a complex surgical procedure.

The current bioprosthetic valves that are available for clinical use inevitably degrade over time due to graft-versus-host reactions to the xenogeneic porcine or bovine tissues13. Valve-related calcification, degradation, and insufficiency could necessitate repeated interventions after several years, especially in young patients who would need to undergo multiple pulmonary valve replacements in their lifetime due to the lack of growth of the valves, a property inherent to current bioprosthetic materials14. Furthermore, the currently available, essentially non-regenerative, TPVR valves have major limitations such as thromboembolic and bleeding complications, as well as limited durability due to adverse tissue remodeling which could lead to leaflet retraction and universal valvular dysfunction15,16.

It is hypothesized that developing a native-like autologous pulmonary valve (APV) mounted onto a self-expandable Nitinol stent for TPVR with the characteristics of self-repair, regeneration, and growth capacity would ensure physiological performance and long-term functionality. And the non-toxic crosslinker treated autologous pericardium can awake from the harvesting and manufacturing procedures. To this end, this preclinical trial was conducted to implant a stented autologous pulmonary valve in an adult sheep model with the aim of developing ideal interventional valvular substitutes and a low-risk procedural methodology to improve the transcatheter therapy of RVOT dysfunction. In this paper, sheep J was selected to illustrate the comprehensive TPVR procedure including pericardiectomy and trans jugular vein implantation of an autologous heart valve.

Protocol

This preclinical study approved by the legal and ethical committee of the Regional Office for Health and Social Affairs, Berlin (LAGeSo). All animals (Ovis aries) received humane care in compliance with the guidelines of the European and German Laboratory Animal Science Societies (FELASA, GV-SOLAS). The procedure is illustrated by performing autologous pulmonary valve replacement in a 3-year-old, 47 kg, female sheep J.

1. Preoperative management

  1. House all experimental sheep in the same room containing straw for 1 week from the day of arrival to the pericardiectomy day to maintain social companionship (Figure 1A).
  2. Deprive the sheep of food but not water for 12 h prior to the pericardiectomy and implantation.
  3. Pre-medicate the sheep with an intramuscular injection of midazolam (0.4 mg/kg), butorphanol (0.4 mg/kg), and glycopyrrolate (0.011 mg/kg or 200 mcg) 20 min before intubation.

2. Induction of general anesthesia

  1. Aseptically place an 18 G safety intravenous (IV) catheter, an injection port, and a T port in the cephalic vein (Figure 1B).
  2. Induce anesthesia by intravenous injection of propofol (20 mg/mL, 1–2.5 mg/kg) and fentanyl (0.01 mg/kg) to effect.
  3. Indications of an adequate level of sedation include jaw relaxation, loss of swallowing, and papillary reflex. After sedation, intubate the sheep with an appropriately sized endotracheal tube (Figure 1C). Shave the sheep and then transfer it to the operating room (OR).

3. Intra-operative anesthesia management for pericardiectomy and implantation

  1. Use a pressure-cycled mechanical ventilator to initiate intermittent positive pressure ventilation (IPPV) with 100% oxygen in the OR.
  2. Connect the sheep to the anesthetic device platform and ventilate the sheep throughout the anesthesia under pressure mode (tidal volume (TV) = 8-12 mL/kg, respiratory frequency (RF) = 12-14 breaths/min). Adjust the TV and RF to keep the end-tidal carbon dioxide (EtCO2) between 35-45 mmHg and the arterial partial pressure of CO2 (PaCO2) below 50 mmHg.
  3. Maintain anesthesia combined with isoflurane (to effect, suggested maintenance concentration 1.5%-2.5%) in oxygen with a flow rate of 1 L/min (inspired fraction of oxygen (FiO2) = 75%), combined with a continuous rate infusion (CRI) of fentanyl (5-15 mcg/kg/h) and midazolam (0.2-0.5 mg/kg/h).
  4. Place an 18 G safety IV catheter in the auricular artery for the measurement of invasive blood pressure (IBP).
  5. Connect the sheep to the multi-function anesthesia platform for hemodynamic monitoring, which displays the direct measurement of invasive blood pressure (IBP) in the auricular artery (zeroed at the level of the heart), body temperature with a rectal probe, a lead-IV electrocardiogram, plethysmographic oxygen saturation (SpO2), TV, RF, EtCO2, heart rate (HR), and FiO2.
  6. Position a gastric tube to evacuate excess gas and fluids from the reticulorumen in preparation for the pericardiectomy. Equip the gastric tube with a marker guide wire as a reference for the implantation.
  7. Place a foley urinary catheter via the urethra inside the bladder connected to a urine bag. Distend the foley balloon with a minimum of 5 mL of saline solution (0.9% NaCl).
  8. Carry out an activated coagulation test (ACT: 240-300 s) 30 min before implantation to confirm sufficient heparinization before and antagonization after the implantation. Perform arterial blood gas analysis (ABGs) to analyze the internal environment 30 min prior to pericardiectomy and implantation and every hour during the two procedures.
  9. Administer the following antibiotics, namely, sulbactam/ampicillin (20mg/kg) 30 min via intravenous drip prior to pericardiectomy and implantation. Ensure a continuous infusion of crystalloids (5 mL/kg/h, isotonic balanced electrolyte solution) and hydroxyethyl starch (HES, 30 mL/h) throughout the pericardiectomy and implantation.

4. Pericardiectomy

  1. Preparation for pericardiectomy
    1. Place the sheep on the operating table in the right lateral recumbent position with 30° elevation on the left side, and then secure her limbs with harnesses and straps.
    2. Sterilize the surgical site (pericardiectomy: superiorly to the left clavicle, anteriorly to the sternum, inferiorly to the level of the diaphragm, and posteriorly to the left midclavicular line) with chlorhexidine-alcohol before performing the minithoracotomy. Cover the remaining areas with sterile draping (Figure 2A).
    3. Make a 5 cm skin incision at the fourth intercostal parasternal position using a #10 surgical blade under general anesthesia.
    4. Dissect the pectoralis major- pectoralis minor- anterior serratus-intercostal muscle via the left lateral minithoracotomy (m-LLT) into 5 cm incisions in length consecutively and separately in the third and fourth intercostal space for ideal exposure (Figure 2B).
    5. Make the incision at least 2 cm offset from the sternum to prevent injury to the left internal thoracic artery and veins. Cease the ventilator for 10 s to prevent lung injury before opening the thorax.
    6. Use several sterile gauzes to compress the left lung for better exposure of the surgical field after placing a rib spreader (Figure 2C). Visualize the pericardium and thymus in the surgical field (Figure 2D).
  2. Start the pericardiectomy at the attachment point of the pericardium and diaphragm and harvest the pericardial tissue between the two phrenic nerves, up to the innominate veins, down to the diaphragm.
    1. Compress the left lung as mentioned in step 4.1.5 to expose the attachment of the diaphragm-pericardium-mediastinal pleura. Cut open the left mediastinal pleura at the attachment of the diaphragm-pericardium-mediastinal pleura by making a 1 cm incision in length using a surgical scissor. Extend the incision upward into the innominate veins along the line which is 1 cm offset from the left phrenic nerve (Figure 2E).
    2. Repeat the procedure for the right part of the pericardium by elevating the apex to the left using fingers. Dissect the thymic and pericardial fat from the sternum.
    3. Meet the two incisions of the pericardium in front of the aorta. Cross clamp the intersection of pericardium and thymus from the two pericardial incisions in front of the aorta by holding them firmly in place and tying six surgical knots manually using a 4-0 non-resorbable suture.
    4. Avoid injury of the phrenic nerve and the underlying vascular structures, when harvesting the pericardium. Dissect adipose tissue including the thymus from the surface of the pericardium during pericardiectomy. Use a cautery tool (i.e., electrotome, Bovie) for hemostasis.
  3. Place the harvested pericardium onto the sterile plate with a centimeter scale to remove the extra adipose tissue, and then wash it twice in 0.9% NaCl (Figure 2F). Double-check all the surgical areas for hemostasis.
  4. Suture the opened right mediastinal pleura to the residual right pericardial edge with 3-0 polydioxanone in a running fashion twice. Inflate the right lung to the largest volume manually using a breathing bag and hold for 10 s prior to closing the right thorax. Suture the opened left mediastinal pleura to the residual left pericardial edge with 3-0 polydioxanone in a running fashion twice.
  5. Close the left thoracic incisions in four layers as described below.
    1. Suture the intercostal muscles and anterior serratus with 2- 0 polydioxanone in a simple interrupted or cruciate fashion, pectoralis major-pectoralis minor with 3-0 polydioxanone in a running fashion, the subcutis with 3-0 polydioxanone in a cruciate fashion, and the skin with 3-0 nylon in a simple interrupted fashion. Place all the sutures at 1 cm intervals.
    2. Inflate the left lung to the largest volume manually using a breathing balloon and hold for 10 s prior to closing the intercostal muscles.
  6. Cover the incision with sterile gauze and compress it manually for 5 min to prevent hemorrhage after heparinization for the new heart valve implantation. Then bandage the surgical site.
  7. Stop the intravenous anesthetics and isoflurane when performing the skin suture to reduce the depth of sedation.
  8. Remove the gastric tube and urinary catheter after the spontaneous respiration returns. Then transfer the sheep with pulse oximetry to the recovery room on the stretcher.
  9. Remove the endotracheal tube when the swallowing reflex, the papillary reflex, and normal spontaneous breathing recover. Administer 0.5 mg/kg meloxicam subcutaneously once a day before the implantation.
  10. Once the anesthesia is completely reversed (i.e., when the sheep is able to stand independently), the sheep can be given access to food and water.

5. Preparation of the three-dimensional autologous heart valve

  1. Trim the pericardium by removing the adipose tissue (Figure 3A,B,C), and then place it onto the 3D shaping heart valve mold. (Due to a pending patent application, figures cannot be provided in this step.)
  2. Put the pericardium and the 3D shaping heart valve model into an incubator with a non-toxic crosslinker (30 mL) for 2 days and 21 h (Figure 3D; due to the pending patent application, figures and detailed information of non-toxic crosslinker cannot be provided in this step).

6. Preparation of the APV

  1. Wash the crosslinked heart valve in 0.9% NaCl twice and suture it into a Nitinol stent (30 mm in diameter, 29.4 mm in height, 48 rhombic cells) in a discontinuous fashion after 2 days and 21 h. Use 5-0 polypropylene to suture the heart valve in place using six to eight knots to align the attachment points between the heart valve and stent. (Due to a patent application, figures cannot be provided in this step.)
  2. Cut the three free edges of the autologous pulmonary valve open with a no. 15 surgical blade (Figure 4A,B). Hold the stented pulmonary valve with a surgical tweezer, lift and leave the APV in 0.9% NaCl to test its opening and closing and to evaluate whether the three commissures need further cutting to achieve a larger opening of the orifice.
  3. Incubate the APV in an incubator for 30 min for sterilization in 47.6 mL of PBS with 0.8% amphotericin B (0.4 mL) and 4.0% penicillin/streptomycin (2 mL). Crimp the stented heart valve into the head of a delivery system (DS) using a commercial crimper for two-fold testing (Figure 4C-D) and fit it into the delivery system (Figure 4E).

7. Transcatheter autologous pulmonary valve implantation via the left jugular vein

  1. Anesthetize the sheep for APV implantation as illustrated in steps 1 to 3.
  2. Vessel access: Shave the sheep and sterilize the surgical field, which includes superiorly to the inferior border of the mandible, anteriorly to the anterior median line, inferiorly to the superior border of left clavicle, and posteriorly to the posterior median line using a povidone-iodine antiseptic before performing the implantation. Cover the remaining unshaved and unsterilized areas with sterile draping.
    1. Mark the left jugular vein on the neck and using the Seldinger technique place the guidewire into the left jugular vein. Enlarge the puncture point with a no. 10 blade, place a 11 F sheath into the left jugular vein for the ICE probe and delivery system (Figure 5A,B). Place a purse-string suture around the sheath introducer with a 4-0 non-absorbable suture.
  3. Intracardiac echocardiography (ICE)17
    1. Perform ICE before and immediately after the implantation using a 10 Fr ultrasound catheter (Figure 5C). Asses the parameters including the dimensions and functions of the NPV, APV, and tricuspid valve by 2D, color, pulsed wave, and continuous Doppler in the short and long axis.
    2. Evaluate the degree of valvular regurgitation in the vena contracta by semi-quantitative assessment18 via ICE (Figure 6).
  4. Angiography19: Perform angiography using a portable C-arm and a functional screen to guide the implantation by measuring the diameters of the RVOT, NPV, pulmonary bulb, and supravalvular pulmonary artery, as well as to evaluate the APV after implantation (Figure 7A-D).
  5. Hemodynamics20: Measure and record the right ventricular and pulmonary artery pressure before and after the implantation using a 5.2 F 145° pigtail catheter. Measure the systemic arterial pressure via the auricular artery.
  6. Implantation
    1. Establishment of the TPVR tract: Place a 0.035-inch angled guidewire to the right pulmonary artery under the guidance of fluoroscopy. Then, place a 5.2 Fr pigtail catheter into the left jugular vein and advance it into the right pulmonary artery with the guidance of the previously placed guidewire under fluoroscopy.
    2. Retrieve the angled guidewire out of the left jugular vein. Place a 5 Fr Berman angiographic balloon catheter into the left jugular vein and advance it into the right pulmonary artery using the guidance of the guidewire.
    3. Pre-shape the 0.035-inch ultra-stiff guidewire into a circle of about 8-10 cm in length with a diameter equaling the distance from the central point of the tricuspid valve to the central point of the pulmonary valve according to the fluoroscopy measurement and advance it into the right pulmonary artery under the guidance of the balloon catheter (Figure 8A). Ensure the wire does not interfere with the tricuspid valve chordae.
    4. Dilate the skin with a no. 11 blade and dilate the left jugular vein using commercial dilators from 16 Fr to 22 Fr sequentially (Figure 8B). Close the incision with a 3-0 polydioxanone purse-string suture after dilatation (Figure 8C). Perform angiography to ensure the desired position of the stent-bearing part of the DS as described in19.
    5. Mark the sinotubular junction of the pulmonary valve at the end-systolic and end-diastolic cardiac phases during pulmonary angiography as the distal border of the landing zone and the basal plane of the pulmonary valve as the proximal border of the landing zone.
    6. Reopen and inspect the stented autologous valve for crimping-induced damage. Re-crimp the APV and fit it into the head of the DS (Figure 8D). Advance the loaded DS via the pre-shaped guidewire through the right ventricular inflow tract (RVIT) and the RVOT to the NPV position (Figure 8E,F, and Figure 9A).
    7. Retract the cover tube of the DS and deploy the APV slowly and directly over the NPV in the landing zone at the end of the diastolic phase under fluoroscopic guidance (Figure 9A-C). Exercise caution when loaded DS is crossing the junction between the RVIT and the RVOT in order to prevent myocardial injury and ventricular fibrillation. The optimal position for the APV is when the middle part of the stent is placed onto the NPV.
    8. Retract the tip of the DS carefully into the cover tube after deployment and retrieve the DS from the sheep (Figure 9D). Repeat ICE (Figure 6D-F), angiography (Figure 7C-D), and hemodynamic measurements for post-examination of the dimensions and functions of the implanted APV. Close the incision on the left side of the neck with the pre-placed purse-string suture and compress it manually.

8. Peri-implantation medication

  1. Prior to implantation, administer the sheep with heparin at a dose of 5000 IU to maintain an activated clotting time (ACT) of 240-300 s. Use ACT tests throughout the procedure. Repeat ACT tests every 30 min after the start of the procedure to confirm both sufficient heparinization before and antagonization after the implantation.
  2. Before the APV implantation, administer 10% magnesium at a dose of 0.02 mol/L and amiodarone at a dose of 3-5 mg/kg to prevent cardiac arrhythmias.
  3. Administer sulbactam/ampicillin (20 mg/kg) intravenously to prevent infection and endocarditis at the start of the pericardiectomy and implantation procedure.

9. Postoperative management

  1. Perform a daily postoperative follow-up for 5 days, checking the general condition of the sheep in terms of heart rate and rhythm, breathing depth, breathing rhythm, and breath sound (for checking postoperative pneumonia), signs of pain, and other abnormalities. Check the wound for postoperative swelling, inflammation, redness, bleeding, and secretion.
  2. Continue anticoagulation for 5 days with dalteparin 5000 IU or another low-molecular-weight heparin administered subcutaneously once daily. Administer 1 mg/kg meloxicam by subcutaneous injection for postoperative analgesia for 5 days.
  3. Perform a laboratory blood test, including hematology, liver function, renal function, and serum chemistry to evaluate the sheep's physical condition.

10. Follow-up

  1. Perform ICE, cardiac magnetic resonance imaging (cMRI), angiography, and record hemodynamics every 3-6 months after implantation for up to 21 months. Perform ICE and angiography as illustrated above.
  2. Perform cMRI to evaluate the regurgitation fraction (RF) on a 3.0 T MRI scanner using a standard electrocardiogram-gated cine-MRI method21. Perform final cardiac computed tomography (CT) to evaluate the stent position and the deformation of the right heart throughout the entire cardiac cycle as illustrated in our previous study22.

Results

In sheep J, the APV (30 mm in diameter) were successfully implanted in the "landing zone" of the RVOT.

In sheep J, the hemodynamics remained stable throughout the left anterolateral minithoracotomy under general anesthesia with ventilation, as well as in the follow-up MRI and ICE (Table 1, Table 2, and Table 3). Autologous pericardium measuring 9 cm x 9 cm was harvested and trimmed by removing extra tissue (Figure ...

Discussion

This study represents an important step forward in developing a living pulmonary valve for TPVR. In an adult sheep model, the method was able to show that an APV derived from the sheep's own pericardium can be implanted with a self-expandable Nitinol stent via jugular vein catheterization. In sheep J, the stented autologous pulmonary valve was successfully implanted in the correct pulmonary position using a self-designed universal delivery system. After implantation, the heart valve of sheep J showed go...

Disclosures

The authors have no financial conflicts of interest to disclose.

Acknowledgements

We extend our heartfelt appreciation to all who contributed to this work, both past and present members. This work was supported by grants from the German Federal Ministry for Economic Affairs and Energy, EXIST - Transfer of Research (03EFIBE103). Yimeng Hao is supported by the China Scholarship Council (CSC: 202008450028).

Materials

NameCompanyCatalog NumberComments
10 % MagnesiumInresa Arzneimittel GmbHPZN: 000911260.02 mol/ L, 10X10 ml
10 Fr Ultrasound catheterSiemens Healthcare GmbHSKU  10043342RHACUSON AcuNav™ ultrasound catheter
3D SlicerSlicerSlicer 4.13.0-2021-08-13Software: 3D Slicer image computing platform
Adobe IllustratorAdobeAdobe Illustrator 2021Software
AmiodaroneSanofi-Aventis Deutschland GmbHPZN: 45993823- 5 mg/ kg, 150 mg/ 3 ml
Amplatz ultra-stiff guidewireCOOK MEDICAL LLC, USAReference Part Number:THSF-35-145-AUS0.035 inch, 145 cm
Anesthetic device platformDrägerwerk AG & Co. KGaA8621500Dräger Atlan A350
ARROW Berman Angiographic Balloon CatheterTeleflex Medical Europe LtdLOT: 16F16M00705Fr, 80cm (X)
ButorphanolRichter Pharma AGVnr5319430.4mg/kg
C-ArmBV Pulsera, Philips Heathcare, Eindhoven, The NetherlandsCAN/CSA-C22.2 NO.601.1-M90Medical electral wquipment
Crimping toolEdwards Lifesciences, Irvine, CA, USA9600CRCrimper
CTSiemens Healthcare GmbHCT platform
DilatorEdwards Lifesciences, Irvine, CA, USA9100DKSA14- 22 Fr
Ethicon SutureEthiconLOT:MKH2594- 0 smooth monophilic thread, non-resorbable
Ethicon SutureEthiconLOT:DEE2743-0, 45 cm
Fast cath hemostasis introducerST. JUDE MEDICAL Minnetonka MNLOT Number: 345829711 Fr
FentanylJanssen-Cilag Pharma GmbHDE/H/1047/001-0020.01mg/kg
FragminPfizer Pharma GmbH, Berlin, GermanyPZN: 5746520Dalteparin 5000 IU/ d
Functional screenBV Pulsera, Philips Heathcare, Eindhoven, The NetherlandsSystem ID: 44350921Medical electral wquipment
GlycopyrroniumbromidAccord Healthcare B.VPZN116491230.011mg/kg
Guide Wire MTERUMO COPORATION JAPANREF*GA35183M0.89 mm, 180 cm
Hemochron Celite ACTInternational Technidyne Corporation, Edison, USANJ 08820-2419ACT
HeparinMerckle GmbHPZN: 3190573Heparin-Natrium 5.000 I.E./0,2 ml
Hydroxyethyl starch (Haes-steril 10 %)Fresenius Kabi Deutschland GmbHATC Code: B05A500 ml, 30 ml/h
Imeron 400 MCTBracco ImagingPZN002299782.0–2.5 ml/kg, Contrast agent
IsofluraneCP-Pharma Handelsges. GmbHATCvet Code: QN01AB06250 ml, MAC: 1 %
Jonosteril InfusionslösungFresenius Kabi Deutschland GmbHPZN: 5416121000 ml
KetamineActavis Group PTC EHFART.-Nr. 799-7622–5 mg/kg/h
MeloxicamBoehringer Ingelheim Vetmedica GmbHM21020A-0920 mg/ mL, 50 ml
MidazolamHameln pharma plus GMBHMIDAZ501000.4mg/kg
MRIPhilips HealthcareIngenia Elition X, 3.0T
Natriumchloride (NaCl)B. Braun Melsungen AGPZN /EAN:04499344 / 40305390773610.9 %, 500 ml
Pigtail catheterCordis, Miami Lakes, FL, USAREF: 533-534A5.2 Fr 145 °, 110 cm
PropofolB. Braun Melsungen AGPZN 1116449520mg/ml, 1–2.5 mg/kg
PropofolB. Braun Melsungen AGPZN 1116444310mg/ml, 2.5–8.0 mg/kg/h
Safety IV Catheter with Injection portB. Braun Melsungen AGLOT: 20D03G834618 G Catheter with Injection port
Sulbactam- ampicillinPfizer Pharma GmbH, Berlin, GermanyPZN: 48431323 g, 2.000 mg/ 1.000 mg
Sulbactam/ ampicillinInstituto Biochimico Italiano G Lorenzini S.p.A. – Via Fossignano 2, Aprilia (LT) – ItalienATC Code: J01CR0120 mg/kg, 2 g/1 g
Surgical BladeBrinkmann Medical ein Unternehmen der Dr. Junghans Medical GmbHPZN: 35484415 #
Surgical BladeBrinkmann Medical ein Unternehmen der Dr. Junghans Medical GmbHPZN: 35484411 #
SutureJohnson & JohnsonHersteller Artikel Nr. EH7284H5-0 polypropylene

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