mRNA Transcription of Transcription Activator-like Effector Nucleases
2:18
Inject Astyanax mexicanus Embryos with TALEN mRNA
4:06
Phenotype Founder Fish and Evaluate TALEN Efficiency
5:27
Results: Germline Transmission of TALEN-induced oca2 Mutations in A. mexicanus
6:47
Conclusion
Transkript
The overall goal of this procedure is to introduce mutations into specific genes in the cave fish Astyanax mexicanus. This method can help answer key questions in the evolutionary biology field about the genetic basis of the evolution of cave trai
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Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.