Traditional Method of Isolating Bone Marrow-derived Cells
2:40
Improved Method of Isolating Bone Marrow-derived Cells
4:11
Purification by Density Gradient Centrifugation
5:39
Culture and Differentiation
6:15
Bone Resorption Assay Using Toluidine Blue Staining
8:03
Scanning Electron Microscopy
9:03
Results: Analysis of the Osteoclast Precursors Isolated, Purified, and Differentiated from Rat Bone Marrow
10:40
Conclusion
Transkript
This method is significant because it can be used to obtain large amounts of fully differentiated osteoclasts in vitro. The main advantage of this method is that it is more stable and safe and isolates bone marrow in less time and with less effort
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Osteoclasts are tissue-specific macrophage polykaryons derived from the monocyte-macrophage lineage of hematopoietic stem cells. This protocol describes how to isolate bone marrow cells so that large quantities of osteoclasts are obtained while reducing the risk of accidents found in traditional methods.