Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids

1:01

Fractionation of LDL (Low-density Lipoprotein) Containing Plasma from Chicken Egg Yolk

2:04

Isolation of LDL Particles from Plasma

3:16

Incubation of S. aureus with LDLs for Membrane Lipid Analysis

5:18

Extraction of S. aureus Membrane Lipids

6:54

Analysis of S. aureus Lipid Profiles using High Resolution/Accurate Mass Spectrometry

8:26

Database Searching to Identify Endogenous S. aureus and Exogenous LDL-derived Lipids

9:54

Results: Fatty Acid Composition of S. aureus Phospholipids Cultured with Chicken LDLs

11:29

Conclusion