Results: Morphological Changes in M. marinum Infection Induced Macrophage Lytic Cell Death and UV Induced Macrophage Apoptosis
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Conclusion
Transkript
This protocol can clearly differentiate macrophage cell death modes during mycobacterial infection. By observing multiple embryos in parallel, it greatly increases the probability of capturing the entire macrophage lytic cell death process. This p
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This protocol describes a technique for visualizing macrophage behavior and death in embryonic zebrafish during Mycobacterium marinum infection. Steps for the preparation of bacteria, infection of the embryos, and intravital microscopy are included. This technique may be applied to the observation of cellular behavior and death in similar scenarios involving infection or sterile inflammation.