Density Gradient Centrifugation to Enrich Lymphocytes from the Digested Kidney
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Results: Flow Cytometry Analysis of Kidney Lymphocytes
5:40
Conclusion
Transkript
CD8+T cells carry distinct features in different tissue, as a critical example of non-mucosal and non-lymphoid tissue. It is essential to directly characterize kidney-resident cells to fully understand T-cell biology. Here we will demonstrate our
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Following viral infection, kidney harbors a relatively large number of CD8+ T cells and offers an opportunity to study non-mucosal TRM cells. Here, we describe a protocol to isolate mouse kidney lymphocytes for flow cytometry analysis.