Described in this video is a protocol for creating and using dry macroporous alginate scaffolds known as Drydux that efficiently promote transduction of activated T-cells. Drydux is designed to be used in place of gold standard spinoculation of Re
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Herein is a protocol for creating dry macroporous alginate scaffolds that mediate efficient viral gene transfer for use in genetic engineering of T cells, including T cells for CAR-T cell therapy. The scaffolds were shown to transduce activated primary T cells with >85% transduction.