Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli

6.4K Views

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05:19 min

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June 30th, 2023

DOI :

10.3791/65442-v

June 30th, 2023

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Bree R. Streather¹, Karen Baker¹, Tara A. Eastwood¹, Daniel P. Mulvihill¹

¹School of Biosciences, University of Kent

Transcript

Summary

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Membrane bound Vesicles

Downstream Processing

Toxic Proteins

Insoluble Proteins

Disulfide Bonds

Biotechnology Applications

Discovery Science

Chapters in this video

0:00

Introduction

0:58

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

3:43

Visualizing Recombinant Protein‐Filled Vesicles from E. coli by Fluorescence Microscopy

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