Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

974 Views

•

05:04 min

•

October 13th, 2023

DOI :

10.3791/65962-v

October 13th, 2023

•

Roy Cohen¹^,², Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis¹^,²

¹Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University

Transcript

Summary

Explore More Videos

Real time Imaging

Acrosomal Calcium Dynamics

Acrosome targeted Sensor For Exocytosis

GCaMP

MCherry Fusion Protein

High Frame rate Imaging

Gene editing CRISPR

Sperm Capacitation

Physiological Conditions

Chapters in this video

0:00

Introduction

0:44

Collection and Washing of Sperm from the AcroSensE Mouse Model for Real‐Time Imaging

2:06

Setting up the Single Cell Stimulant Delivery System for Real‐Time Monitoring of Live Mouse Sperm

3:21

Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse

Related Videos

10:10

Observing Mitotic Division and Dynamics in a Live Zebrafish Embryo

11.9K Views

10:03

Bioengineering of Humanized Bone Marrow Microenvironments in Mouse and Their Visualization by Live Imaging

11.6K Views

05:41

In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

10.8K Views

06:10

Live Imaging of Mouse Secondary Palate Fusion

7.5K Views

10:13

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

7.5K Views

08:33

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae

11.3K Views

11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

8.7K Views

10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

7.5K Views

06:10

Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

2.2K Views

11:41

Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

1.9K Views