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Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

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00:46 min

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October 13th, 2023

DOI :

10.3791/65962-v

October 13th, 2023

•

628 Views

Roy Cohen¹^,², Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis¹^,²

¹Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University

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Real time Imaging

Acrosomal Calcium Dynamics

Acrosome targeted Sensor For Exocytosis AcroSensE

Genetically Encoded Calcium Indicator GCaMP

High Frame rate Imaging

Sperm Activation

Membrane Fusion

CRISPR Gene editing

Transgenic Mouse Model

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