Methods Article

Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

720 Views

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05:04 min

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October 13th, 2023

DOI :

10.3791/65962-v

October 13th, 2023

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Roy Cohen¹^,², Danielle M. Sosnicki¹, Melissa A. White¹, Jacquelyn L. Nelson¹, Chinatsu Mukai¹, Alexander J. Travis¹^,²

¹Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, ²Department of Public and Ecosystem Health, College of Veterinary Medicine, Cornell University

Transcript

Summary

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Real time Imaging

Acrosomal Calcium Dynamics

Acrosome targeted Sensor For Exocytosis

GCaMP

MCherry Fusion Protein

High Frame rate Imaging

Gene editing CRISPR

Sperm Capacitation

Physiological Conditions

Chapters in this video

0:00

Introduction

0:44

Collection and Washing of Sperm from the AcroSensE Mouse Model for Real‐Time Imaging

2:06

Setting up the Single Cell Stimulant Delivery System for Real‐Time Monitoring of Live Mouse Sperm

3:21

Combining Microscopy with a Stimulant Delivery System to Monitor Live Sperm from an AcroSensE Model Mouse

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