Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

1.5K Views

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09:43 min

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March 8th, 2024

DOI :

10.3791/66492-v

March 8th, 2024

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Aline Linder*¹, Kevin Portmann*¹, Luca Johannes Schlotheuber¹, Alessandro Streuli¹, Wiona Sophie Glänzer¹, Klaus Eyer¹^,², Ines Lüchtefeld¹^,³

¹Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, ²Department of Biomedicine, Aarhus University, ³Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology, ETH Zürich

Summary

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Microfluidic Approach

Cytokine Secretion

Polyfunctional Cells

Single cell Resolution

Quantitative Analysis

Immune Response Dysregulation

Cellular Functionality

Cytokine Dynamics

Dynamic Range Assay

Immune Cell Activation

Measurement Protocol

Analytical Strategies

Immune Cell Heterogeneity

Time resolved Measurement

Chapters in this video

0:00

Introduction

2:04

Observation Chamber Fabrication for Imaging Single‐Cell Suspensions and Magnetic Nanoparticles

3:50

Preparation of Functionalized Magnetic Nanoparticles for Time‐Resolved Fluorescent Imaging

5:33

Encapsulation of the Cells and Nanoparticles into Droplets for Fluorescent Imaging

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