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Ex Vivo Culture of Circulating Tumor Cells in the Cerebral Spinal Fluid from Melanoma Patients to Study Melanoma-Associated Leptomeningeal Disease

701 Views

06:25 min

March 29th, 2024

DOI :

10.3791/66071-v

March 29th, 2024


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Ex Vivo Culture

Chapters in this video

0:00

Introduction

1:36

Preparation of Human Meningeal Cell (HMC) Conditioned Media and Collection of Cerebrospinal Fluid for the Culture of Circulating Tumor Cells

3:23

In Vitro Culture and Expansion of Cerebro Spinal Fluid (CSF)-Circulating Tumor Cells (CTCs) for the Study of Leptomeningeal Disease

4:24

CSF Collection from Mice with Leptomeningeal Disease for Subsequent Clone Expansion

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