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Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

DOI :

10.3791/53202-v

September 30th, 2015

September 30th, 2015

9,280 Views

1Department of Neuroscience, Biomedical Center, Uppsala University

This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.

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