April 9th, 2011
•Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.
Tags
Related Videos
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
Flow Cytometry Analysis of Immune Cells Within Murine Aortas
Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
Examination of Thymic Positive and Negative Selection by Flow Cytometry
Quantitative Measurement of the Immune Response and Sleep in Drosophila
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved