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Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

DOI :

10.3791/2724-v

June 16th, 2011

June 16th, 2011

13,176 Views

1Departments of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida

Cell-mediated lymphocytotoxicity (CML) assays can be used to test autoreactive responses and study mechanisms of cell death in vitro. However, using live-cell confocal microscopic imaging techniques with fluorescent dyes, the type and kinetics of cell death as well as the pathways utilized can be studied in greater detail.

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