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Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

DOI :

10.3791/3054-v

9:45 min

July 15th, 2011

July 15th, 2011

16,322 Views

1Institut Cochin, Université Paris Descartes, CNRS (UMR 8104), 2Inserm, U1016, Paris, France

This protocol describes a method to image fluorescent T cells introduced into lymph node slices. The technique permits real-time analyses of T cell migration with traditional widefield fluorescence or confocal microscopes.

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Ex Vivo Imaging

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