JoVE Logo
Faculty Resource Center

Sign In

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells

DOI :

10.3791/3738-v

April 16th, 2012

April 16th, 2012

23,620 Views

1Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky

We describe a method for generating regulatory, memory and naïve T cells from a single human blood donor. Polarized Tregs can be then compared to other subsets in a variety of genetic and functional applications with genetic homogeneity, including a suppression assay also detailed here.

-- Views

Related Videos

article

Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro

article

Retroviral Transduction of T-cell Receptors in Mouse T-cells

article

Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

article

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice

article

Competitive Homing Assays to Study Gut-tropic T Cell Migration

article

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays

article

Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method

article

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

article

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant

article

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved